Inhibin alpha Antibody

SKU: orb1823741

Description

It recognizes a 47kDa protein, which is identified as alpha sub-unit of Inhibin. It is a gonadal protein that preferentially suppresses the secretion of pituitary follicle-stimulating hormone (FSH). Inhibin comprises two subunits, Inhibin A and Inhibin B. Each subunit consists of the same Alpha subunit, covalently linked to 1 of 2 distinct subunits, beta-Alpha or beta-beta. Originally isolated from ovarian follicular fluid and characterized as a disulphide-linked dimeric glycoprotein, inhibin belongs to the transforming growth factor beta (TFGbeta) superfamily. Antibodies against Inhibin are useful in making a differentiation between adrenal cortical tumors and renal cell carcinoma. Sex cord stromal tumors of the ovary as well as trophoblastic tumors also demonstrate cytoplasmic positivity. Inhibin antibody is also used to make the differential diagnosis of intra-uterine vs. ectopic pregnancy in endometrial curetting.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Inhibin alpha antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: INHA/8872R
• Immunogen: A recombinant fragment of human Inhibin alpha protein (within amino acids 233-362) was used as the immunogen for the Inhibin alpha antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human testicular carcinoma tissue with INHA antibody (clone INHA/8872R) at 2 ug/ml. HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC testing of FFPE human brain tissue with INHA antibody (clone INHA/8872R) at 2 ug/ml. HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P05111