INH1

SKU: orb1222375

Description

INH1 is a cell-permeable Hec1 inhibitor that specifically disrupts the Hec1/Nek2 interaction.

Key Properties

• CAS Number: 313553-47-8
• MW: 308.4
• Purity: >98% (HPLC)
• Formula: C₁₈H₁₆N₂OS
• SMILES: Cc1cc(C)c(c2csc(NC(=O)c3ccccc3)n2)cc1
• Target: TNF
• Solubility: DMSO : 100 mg/mL 324.25 mM

Bioactivity

• In Vivo:
  INH1 (50 or 100 mg/kg, i.p. , every other day/25 cycles) inhibits tumor outgrowth in a xenografted breast cancer model in nude mice. Animal model: Xenografted nude mice breast cancer model. Dosage: 50 or 100 mg/kg. Administration: i.p. , every other day/25 cycles. Result: Inhibited tumor growth.
• In Vitro:
  INH1 (25 μM, 24 h) treatment resulted in reduced association of Hec1 with kinetochore and decrease of global Nek2 protein level. INH1 exhibits GI50 values of 10.5 μM (in MDA-MB-468 cells), 15 μM (in SKBR3 cells), 10.5 μM (in T47D cells), 20.5 μM (in MDA-MB-361 cells), 15 μM (in ZR-75-1 cells), 15 μM (in HBL 100 cells), 15.5 μM (in MDA-MB-435 cells), 11 μM (in HS578T cells) and 41 μM (in MCF10A cells), respectively. INH1 (5k) has an IC50 value of 176 nM in the dose-dependent transwell migration assays in MDA-MB-231 cells. INH1 (5k) substantially reduces cellular f-actin and prevented localization of fascin to actin-rich membrane protrusions. INH1 induces abnormal mitotic processes, as well as cell apoptosis. Cell Cytotoxicity Assay Cell line: MCF10A cells. Concentration: 10 μM. Incubation time: 12 days. Result: Effectively inhibits the proliferation of human breast cancer lines. Western blot analysis. Cell line: MCF10A cells. Concentration: 25 μM. Incubation time: 24 h. Result: Nek2 reduction in INH1-treated cells may be independent of Hec1.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IBT13131

Quick Database Links

Gene Symbol

TNF