Infliximab Antibody

SKU: orb1297794

Description

Infliximab

Research Area

Cell Biology, Infectious Disease & Virology

Key Properties

• CAS Number: 170277-31-3
• MW: 149 kDa
• Purity: 99.70%
• Target: Antibacterial,TNF

Bioactivity

• Target IC50:
  solTNF:7 ng/mL|TNF-α:0.78 nM (EC50)|TNF-α:280 pM
• In Vivo:
  METHODS: To detect anti-tumor activity in vivo, ML162 (40 mg/kg once daily) and anti-PD-1 antibody (200 µg every three days) were intraperitoneally injected into BALB/c mice bearing TS/A tumors once daily for two weeks. RESULTS: The combination of GPX4 inhibitor and anti-PD-1 antibody significantly inhibited tumor growth compared to monotherapy. The combination therapy did not result in additional immune cell infiltration compared to monotherapy, but the combination therapy induced a significant immune response with an increased proportion of PRF1+CD8+ T cells and GZMB+CD8+ T cells.
• In Vitro:
  METHODS: Human lung cancer cells A549 were pretreated with ML162 (0.1-15 µM) for 4-24 h. TXNRD1 activity was measured with the RX1 activity probe. RESULTS: RX1 signaling was significantly dose-dependently inhibited at ML162 concentrations of 0.5 µM or higher. there was also a fairly rapid onset of concentration-dependent inhibition of cellular TXNRD1 activity by ML162, and a 4 h incubation with 1 µM or higher ML162 treatment was sufficient to inhibit RX1 signaling. ML162 effectively inhibited cellular TXNRD1 activity. METHODS: Melanoma cells A2058 and A375 were pretreated with ferrostatin (10 µM), Z-VAD-FMK (10 µM), or necrosulfonamide (0.5 µM) for 24 h, and cell viability was measured after ML162 (1-16 µM) treatment. RESULTS: ML162 caused cell death in a dose-dependent manner in the A2058 and A375 melanoma cell lines, which could be reversed by ferrostatin-1, a ferritin-specific inhibitor, but not by Z-VAD-FMK, an inhibitor of apoptosis, or necrosulfonamide, an inhibitor of necroptosis.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

autoimmune, Avakine, anti-inflammatory, diabetic, CT-P13, neuropathy, Infliximab, monoclonal, inhibit, Inhibitor, Tumor Necrosis Factor Receptor, PTP1B, TNF Receptor, TNFR, TNFR2, TNFR1

Quick Database Links

Gene Symbol

Antibacterial,TNF