IM-12

SKU: orb1305870

Description

IM-12 is a potent and selective small molecule inhibitor of GSK-3β with an IC₅₀ of 53 nM. It acts as a canonical Wnt/β-catenin pathway activator and is a valuable chemical tool for studying Wnt signalling in stem cell biology, oncology, and neurological research, both in vitro and in vivo.

Research Area

Stem Cell & Developmental Biology

Key Properties

• CAS Number: 1129669-05-1
• MW: 377.4
• Purity: >98%
• Formula: C₂₂H₂₀FN₃O₂
• SMILES: CN1C(=O)C(NCCc2ccc(F)cc2)=C(C1=O)c1c(C)[nH]c2ccccc12
• Target: Kinase
• Solubility: Soluble in DMSO (50 mg/ml)

Bioactivity

• Target IC50:
  GSK-3β:53 nM
• In Vivo:
  In human neural progenitor cells (hNPCs) with an IC50 of 3.8 μM, IM-12 inhibits GSK-3β activity and increases the concentration of β-catenin. IM-12 promotes cell proliferation in human neural progenitor cells by activating the Wnt signaling pathway.
• Cell Research:
  To measure viable cells, 50-100 μL of cell suspension is analyzed using CASY technology with the appropriate program. ReNcell VM cells are seeded at a defined cell number and proliferated for 24 h. Then the medium is changed to proliferation medium with added substances at indicated concentrations. The cell number was determined every 24 h. Cells were exposed to the added drugs during the whole experiment, whereas the media is changed every 24 h.(Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, Glycogen synthase kinase 3, Glycogen synthase kinase-3, GSK-3β, GSK3, GSK-3, inhibit, IM-12, IM 12, IM12

Compound Identifiers

PubChem CID

25209788