You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1256249 |
---|---|
Category | Antibodies |
Description | IL5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 20-134 of human IL5 (NP_000870.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 13kDa |
Target | IL5 |
UniProt ID | P05113 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | EDF, TRF, IL-5Interleukin-5, B-cell differentiatio Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using IL5 antibody (orb1256249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human liver using IL5 antibody (orb1256249) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using IL5 antibody (orb1256249) at dilution of 1:100 (40x lens).
Filter by Rating