IL3RA/CD123 Antibody

SKU: orb1248078

Description

IL3RA/CD123 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 43kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 43.3kDa according to NP_002174.1). Recommended concentration: 1-3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: RQQYECLHYKTD
• Target: IL3RA / CD123
• Molecular Weight: Approx 43kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IL3RA, interleukin 3 receptor, alpha (low affinity), CD123, IL3R, IL3RAY, IL3RX, IL3RY, hIL-3Ra, CD123 antigen, IL-3 receptor alpha SP2 isoform, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA, interleukin-3 receptor subunit alpha

orb1248078 (2 ug/ml) staining of Jurkat (A) and Molt4 (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248078 (5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

IL3RA / CD123

UniProt

P26951

RefSeq (Protein):NP_001254642.1, NP_002174.1