IL3RA Antibody / CD123

• Catalog Number: orb534469
• Category: Antibodies
• Description: CD123/IL3RA is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CDLA123-1R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A portion of amino acids 26-171 was used as the immunogen for the recombinant IL3RA antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant IL3RA antibody is available for research use only.
• UniProt ID: P26951
• Storage: Store the recombinant IL3RA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The stated application concentrations are suggested starting points. Titration of the recombinant IL3RA antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil with IL3RA antibody (clone CDLA123-1R). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min and allow to cool.

SDS-PAGE analysis of purified, BSA-free IL3RA antibody (clone CDLA123-1R) as confirmation of integrity and purity.