IL25 Antibody (Center)

• Catalog Number: orb1935600
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This IL25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-104 amino acids from the Central region of human IL25.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9H293
• MW: 20330 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB34241
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Interleukin-25, IL-25, Interleukin-17E, IL-17E, IL
• Note: For research use only
• NCBI: NP_758525.1, NP_073626.1

IL25 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35 ug/lane). This demonstrates the IL25 antibody detected the IL25 protein (arrow).

All lanes: Anti-IL25 Antibody (Center) at 1:1000 dilution. Lane 1: human testis lysate. Lane 2: PC-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 20 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

IL25 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of IL25 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.