IL1B Antibody (Center)

SKU: orb1928449

Description

IL1B Antibody (Center)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Immunology & Inflammation, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: FC - 1:25, WB - 1:2000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Monkey, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 30748 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IL1F2

Western blot analysis of IL1B Antibody (Center) in NCI-H460 cell line lysates (35 ug/lane). IL1B (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma with IL1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-IL1B Antibody (Center) at 1:2000 dilution. Lane 1: human spleen lysate. Lane 2: mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 31 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing MCF-7 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

UniProt

P01584