IL18BP Antibody

• Catalog Number: orb1257005
• Category: Antibodies
• Description: IL18BP Antibody
• Target: IL18BP
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 31-194 of human IL18BP (NP_001138529.1).
• UniProt ID: O95998
• MW: Observed: 38kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IL18BPa, interleukin-18-binding protein, MC51L-53L
• Research Area: Immunology & Inflammation
• Note: For research use only

Western blot analysis of extracts of various cell lines, using IL18BP antibody (orb1257005) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse heart using IL18BP antibody (orb1257005) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using IL18BP antibody (orb1257005). Blue: DAPI for nuclear staining.