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Catalog Number | orb1238913 |
---|---|
Category | Antibodies |
Description | IL17RA Antibody |
Target | IL17RA |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse |
Form/Appearance | Liquid |
Concentration | 1 mg/mL |
Buffer/Preservatives | IL-17RA Antibody is supplied in PBS containing 0.02% sodium azide. |
Immunogen | Anti-IL-17RA antibody was raised against a peptide corresponding to 18 amino acid synthetic peptide near the amino terminus of human IL-17RA. The immunogen is located within amino acids 150-200 of IL-17RA. |
UniProt ID | Q96F46 |
MW | Predicted: 96 kD Observed: 100 kD |
Tested applications | ELISA, IF, IHC-P, WB |
Application notes | WB: 1-4 μg/mL; IHC-P/IF: 10-20 μg/mL. Antibody validated: Western Blot in human and mouse samples; Immunofluorescence in human and mouse samples. All other applications and species not yet tested. |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | IL-17RA Antibody: CD217, IL17R, CANDF5, CDw217, IL Read more... |
Note | For research use only |
NCBI | NP_055154 |
WB Validation in K562 Cells. Loading: 10 μg of lysate. Antibodies: IL-17RA, orb1238913, 4 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
WB Validation in Mouse Tissues. Loading: 15 μg of lysate. Antibodies: IL-17RA, orb1238913, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of IL-17RA in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling IL-17RA with orb1238913 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Immunofluorescence Validation of IL-17RA in Mouse Thymus. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus tissue labeling IL-17RA with orb1238913 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
ELISA, FC | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
FC | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |