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Catalog Number | orb1937804 |
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Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB31605 |
Tested applications | IF, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 20437 Da |
Target | This IL17B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-66 amino acids from the Central region of human IL17B. |
UniProt ID | Q9UHF5 |
NCBI | NP_055258.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Interleukin-17B, IL-17B, Cytokine Zcyto7, Interleu Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-IL17B Antibody (Center) at 1:1000 dilution + Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 20 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of IL17B Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
IL17B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of IL17B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
IL17B Antibody (Center) western blot analysis in mouse spleen tissue lysates (35 ug/lane). This demonstrates the IL17B antibody detected the IL17B protein (arrow).
Western blot analysis of IL17B (arrow) using rabbit polyclonal IL17B Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IL17B gene.