IL12RB1 Antibody

SKU: orb1258406

Description

IL12RB1 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:10 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-305 of human IL12RB1 (NP_714912.1).
• Target: IL12RB1
• Molecular Weight: Observed: 73kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IL12RB1, MGC34454, interleukin-12 receptor beta-1 chain, interleukin 12 receptor, beta 1, IL-12R-BETA1, IL12RB, IL-12 receptor beta component, CD212, IL12R

Western blot analysis of extracts of various cell lines, using IL12RB1 antibody (orb1258406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Immunofluorescence analysis of A549 cells using IL12RB1 antibody (orb1258406). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

IL12RB1

UniProt

P42701