IL12B Antibody (C-term)

• Catalog Number: orb1938973
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This IL12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-290 amino acids from the C-terminal region of human IL12B.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P29460
• MW: 37169 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB28444
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Interleukin-12 subunit beta, IL-12B, Cytotoxic lym
• Note: For research use only
• NCBI: NP_002178.2

IL12B Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

IL12B Antibody (C-term) western blot analysis in CEM, MDA-MB453 cell line lysates (35 ug/lane). This demonstrates the IL12B antibody detected the IL12B protein (arrow).

IL12B antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL12B antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.