IL10 Antibody (Center)

• Catalog Number: orb1928106
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This IL10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-53 amino acids from the Central region of human IL10.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Virus
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P22301
• MW: 20517 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB21234
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Interleukin-10, IL-10, Cytokine synthesis inhibito
• Research Area: Cancer Biology, Immunology & Inflammation, Signal
• Note: For research use only
• NCBI: NP_000563.1
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IL10 Antibody (Center) in MDA-MB435 cell line lysates (35 ug/lane). IL10 (arrow) was detected using the purified Pab.

IL10 Antibody (Center) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with IL10 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.