IL-8Rβ (phospho Ser347) rabbit pAb

• Catalog Number: orb768761
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IL8R beta (phospho-Ser347)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human IL-8R beta/CDw128 beta around the phosphorylation site of Ser347. AA range:311-360
• MW: 48
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CXCR2 antibody, anti-IL8RB antibody, anti-C-X
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of 3T3 cells using Phospho-IL-8Rβ (S347) Polyclonal Antibody.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody.

Immunofluorescence analysis of COS7 cells, using IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody. The picture on the right is blocked with the phospho peptide.