Il7 Antibody (Biotin)

SKU: orb1671436

Description

Il7 Antibody (Biotin)

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Rat
• Application Notes:
  Sandwich ELISA: To detect Rat IL-7 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Rat IL-7 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-7. Western Blot To detect Rat IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Rat IL-7 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Rat IL-7. Anti-Rat IL-7 specific antibody was purified by affinity chromatography and then biotinylated.
• Target: Il7
• Purification: IL-7 specific antibody was purified by affinity chromatography and then biotinylated
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lymphopoietin 1(LP-1), pre-B cell factor

To detect Rat IL-7 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat IL-7 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-7.

Quick Database Links

Gene Symbol

Il7

UniProt

P56478

RefSeq:P56478