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IL-6 Recombinant Protein
Description
Research Area
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | The ED50 was determined by the dose - dependent stimulation of the proliferation of IL - 6 - dependent murine 7TD1 cells is 5 x 107 units/mg. |
| Target | Il6 |
| Protein Sequence | MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
Storage & Handling
−| Storage | The lyophilized IL-6 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted IL-6 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing IL-6 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Greater than 95% as determined by SDS-PAGE
22.8 kDa
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1 mg, 100 μg, 20 μgRecombinant Rhesus Macaque IL-6 Protein [orb2992743]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 21.8 KDa. Observed: 22-33 KDa, reducing conditions
1 mg, 500 μg, 50 μg, 10 μgRecombinant Mouse IL-6 Protein [orb2994610]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)
Predicted: 21.8 KDa. Observed: 22 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human IL-6 Protein [orb2995250]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 20.9 KDa. Observed: 20 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_112445.1 |
|---|
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Protocol Information
IL-6 Recombinant Protein (orb1230976)
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