IL-6 Antibody

• Catalog Number: orb1294370
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IL6
• Target: IL-6
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant fragment corresponding to Human IL-6.
• Tested applications: IF, IHC, WB
• Dilution range: Western Blot 1:500-1:700 Immunofluorescence 1:300-1:400 Immunohistochemistry (Paraffin) 1:100-1:200
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Immunology & Inflammation
• Note: For research use only

Anti-IL6 antibody - at 1/500 dilution, Lysates at 60 µg per lane. This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294370 overnight at 4°C.

Anti-IL6 antibody - at 1/500 dilution, Lysates at 60 µg per lane. This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294370 overnight at 4°C.

Cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294370 anti-IL-6 antibody (red) at 1:300 and 4°C.

Immunohistochemical analysis of paraffin embedded Human testis cancer tissue labeling IL-6 with orb1294370 at 1/400.

Immunohistochemical analysis of paraffin embedded Human testis cancer tissue labeling IL-6 with orb1294370 at 1/400.