IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) Antibody

• Catalog Number: orb2309945
• Category: Antibodies
• Description: IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) Antibody
• Target: IL18
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Purification: Protein A
• Immunogen: Recombinant fragment (around aa1-193) of human IL-18 protein (exact sequence is proprietary)
• UniProt ID: Q14116
• MW: 18kDa
• Tested applications: IHC, WB
• Clone Number: IL18/4622
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Iboctadekin; IFN gamma inducing factor; IGIF; IL1
• Research Area: Cardiovascular Research, Cell Biology, Immunology
• Note: For research use only

Analysis of Protein Array containing more than 19000 full-length human proteins using IL-6-Monospecific Mouse Monoclonal Antibody (IL18/4622). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human kidney stained with IL-6 Mouse Monoclonal Antibody (IL18/4622) at 2 ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human liver stained with IL-6 Mouse Monoclonal Antibody (IL18/4622) at 2 ug/ml. HIER: Tris/EDTA, pH9.0, 45 min. 2°C: HRP-polymer, 30 min. DAB, 5 min.

Western blot analysis of A549 lysate using IL-18 Mouse Monoclonal Antibody (IL18/4622).