IL6 Antibody

SKU: orb750682

Description

IL6 antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: DOT, ELISA, IHC, IP, WB
• Dilution Range: ELISA: 1:1,000 - 1:5,000, IHC: 1:400 - 1:800, IP: 1:400 - 1:800, WB: 1:500 - 1:2,000
• Reactivity: Human
• Application Notes:
  IL-6 antibody has been tested in western blot. This antibody is suitable for use in neutralizations, ELISA, radioimmunoassay, and immunoprecipitation. Reactivity in other immunoassays is unknown. Expect ~23.7kDa. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. For immunoblot use the supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10ng/mL E. coli LPS. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. The antiserum is useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.
• Target: IL6
• Purity: Anti-IL-6 antiserum detects recombinant and native human IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). This product has minimal reactivity with mouse IL-6.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: None
• Concentration: 80 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

HSF, Hybridoma growth factor, HGF, Hybridoma plasmacytoma growth factor, IL 6, IL6 protein, IFNB2, Interferon beta 2, BSF2, CDF, B-Cell Differentiation Factor, B-cell stimulatory factor 2, CTL differentiation factor, rabbit anti-Interleukin 6 Antibody, rabbit anti-IL-6

Quick Database Links

Gene Symbol

IL6

UniProt

P05231

RefSeq (Protein):NP_000591.1