IL-6 Antibody

• Catalog Number: orb1272694
• Category: Antibodies
• Description: IL-6 Antibody
• Target: IL6
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL-6. Human IL-6 specific antibody was purified by affinity chromatography employing immobilized hIL-6 matrix.
• UniProt ID: P05231
• Tested applications: ELISA, NeA, WB
• Application notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-6 (5 ng/mL), a concentration of 0.05 - 0.08 μg/mL of this antibody is required.ELISA:Indirect:To detect hIL-6 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6.SandwichTo detect hIL-6 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human IL-6 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6. Western Blot:To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HGF, HSF, BSF2, IL-6, IFNB2, Interleukin-6, B-cell
• Research Area: Immunology & Inflammation, Neuroscience
• Note: For research use only
• NCBI: P05231

To detect Human IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-6 (orb1272693) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-6.

To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.