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Catalog Number | orb749417 |
---|---|
Category | Antibodies |
Description | IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B-cells and T-cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T-cells, B-cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes. |
Species/Host | Rat |
Clonality | Monoclonal |
Clone Number | MP5-20F3 |
Tested applications | FACS, IF, NeA |
Reactivity | Mouse |
Isotype | Rat IgG1, kappa |
Immunogen | Recombinant mouse IL-6 protein was used as the immunogen for this antibody. |
Dilution range | Neutralization Studies (order BSA/sodium azide-free format),Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml |
Purity | Protein G purified IL-6 antibody |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This IL-6 antibody is available for research use only. |
Entrez | 16193 |
Storage | Store the IL-6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the IL-6 antibody to be titered up or down for optimal performance. |
Expiration Date | 12 months from date of receipt. |
Unconjugated | |
90% | |
63.9 kDa | |
Mouse IL-6 R alpha Protein, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Glu 357 (Accession # P22272-1). |
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