Il6 Antibody (Biotin)

SKU: orb1272442

Description

Il6 Antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Rat
• Application Notes:
  ELISA:Sandwich:To detect Rat IL-6 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat IL-6 (XP-5196) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-6.Western Blot:To detect rat IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant rat IL-6 (rat IL-6).
• Target: Il6
• Purification: Anti-rat IL-6 specific antibody was purified by affinity chromatography and then biotinylated.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

ILg6, Ifnb2, Il-6, Interleukin-6IL-6

To detect Rat IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Rat IL-6 (orb1272443) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-6.

Quick Database Links

Gene Symbol

Il6

UniProt

P20607

RefSeq:P20607