You have no items in your shopping cart.
Cart summary
IL-6 Antibody Biotin Conjugated
Catalog Number: orb345172
| Catalog Number | orb345172 |
|---|---|
| Category | Antibodies |
| Description | IL6 antibody (Biotin) |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Biotin |
| Reactivity | Human |
| Form/Appearance | Lyophilized |
| Concentration | 1.0 mg/mL |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Purity | This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human Il-6 in cell supernatants and certain body fluids. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. A control of similarly diluted normal rabbit IgG is recommended. This product has minimal reactivity with mouse IL-6. |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-6 produced in E.coli. |
| UniProt ID | P05231 |
| Tested applications | ELISA, IHC, WB |
| Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
| Application notes | This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. |
| Antibody Type | Primary Antibody |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Alternative names | rabbit Anti-IL-6 biotin conjugated antibody, rabbi Read more... |
| Note | For research use only |
| NCBI | NP_000591.1 |
- Item 1 of 1
- Item 1 of 1
- Item 1 of 1
Mouse Interleukin 6 (IL6) Microsample ELISA Kit [orb1736446]
Mouse
7.82-500 pg/mL
3.1 pg/mL
96 T, 48 T
