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Description
Research Area
Immunology & Inflammation
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | The ED50 was determined by the dose - dependent stimulation of the proliferation of human TF - 1 cells is 2 x 106 units/mg. |
| Target | Il5 |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
| Protein Sequence | MEIPMSTVVK ETLIQLSTHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEILFQNL SLIKKYIDGQ KEKCGEERRK TRHFLDYLQE FLGVMSTEWA MEV |
Storage & Handling
−| Storage | The lyophilized IL-5 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted IL-5 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing IL-5 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Il-5, Interleukin-5, B-cell growth factor II, IL-5
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 13.94 kDa after removal of the signal peptide.The apparent molecular mass of IL5-His is approximately 15-25 kDa due to glycosylation.
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100 μg, 50 μg, 10 μg Recombinant Human IL-5RA Protein [orb2994170]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
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1 mg, 10 μg, 50 μg, 500 μgRecombinant Human IL-5RA Protein [orb2994171]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 62.5 KDa. Observed: 70-85 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Mouse IL-5RA Protein [orb2994722]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 37.6 KDa. Observed: 48 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_068606.1 |
|---|
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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