IL-5 Antibody

• Catalog Number: orb1272635
• Category: Antibodies
• Description: IL-5 Antibody
• Target: Il5
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Murine IL-5. Murine IL-5 specific antibody was purified by affinity chromatography employing an immobilized Murine IL-5 matrix.
• UniProt ID: P04401
• Tested applications: ELISA, WB
• Application notes: ELISA:Sandwich:To detect Murine IL-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-5 (38-283) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-5.Western Blot:To detect Murine IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Murine IL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Il-5, Il-5, Interleukin-5, B-cell growth factor II
• Note: For research use only
• NCBI: P04401

To detect Murine IL-5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-5 (orb1272624) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-5.

To detect Murine IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.