Il5 Antibody (Biotin)

• Catalog Number: orb1272624
• Category: Antibodies
• Description: Il5 Antibody (Biotin)
• Target: Il5
• Clonality: Polyclonal
• Conjugation: Biotin
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Murine IL-5. Murine IL-5 specific antibody was purified by affinity chromatography and then biotinylated.
• UniProt ID: P04401
• Tested applications: ELISA, WB
• Application notes: ELISA:Sandwich:To detect Human IL-17D by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human IL-17D (38-266) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-17D.Western Blot:To detect Human IL-17D by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-17D is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Antibody Type: Primary Antibody
• Storage: The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-80˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles.
• Alternative names: Il-5, Il-5, Interleukin-5, B-cell growth factor II
• Note: For research use only
• NCBI: P04401

To detect Murine IL-5 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti- Murine IL-5 (orb1272635) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine IL-5.