IL4 Antibody

SKU: orb345142

Description

IL4 antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:10,000, WB: 1:1,000
• Reactivity: Human
• Application Notes:
  This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µl by the LAL method. By western blot a band approximately 15 kDa in size corresponding to native human IL-4 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-4 protein.
• Target: IL4
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-4 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store anti-IL-4 antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-IL-4 antibody, rabbit anti-interleukin-4 antibody, BCGF-1, BCGF1, Binetrakin, BSF 1, BSF1, HCGF, IA Inducing Factor, IGG1 induction factor, IL 4, Il4e12, Interleukin 4, Interleukin 4, isoform 1, Interleukin4, Lymphocyte stimulatory factor 1, MCGF2, MFF, Pitrakinra, TCGF2

Quick Database Links

Gene Symbol

IL4

UniProt

P05112

RefSeq (Protein):NP_000580.1