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| Catalog Number | orb1272452 |
|---|---|
| Category | Antibodies |
| Description | IL-4 Antibody |
| Target | Il4 |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Reactivity | Rat |
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Purification | Anti-Rat IL-4 specific antibody was purified by affinity chromatography employing immobilized Rat IL-4 matrix. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat IL-4 (Rat Interleukin-4). |
| UniProt ID | P20096 |
| Tested applications | ELISA, WB |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Il4e12, Il-4, Interleukin-4, B-cell IGG differenti Read more... |
| Research Area | Immunology & Inflammation |
| Note | For research use only |
| NCBI | P20096 |

To detect Rat IL-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Rat IL-4 (orb1272451) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-4.

To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
WB | |
Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
Human | |
0.32-20 ng/mL | |
0.124 ng/mL |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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