Il4 Antibody (Biotin)

• Catalog Number: orb1272451
• Category: Antibodies
• Description: Il4 Antibody (Biotin)
• Target: Il4
• Clonality: Polyclonal
• Conjugation: Biotin
• Reactivity: Rat
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Purification: Anti-rat IL-4 specific antibody was purified by affinity chromatography and then biotinylated.
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat IL-4 (rat Interleukin-4).
• UniProt ID: P20096
• Tested applications: ELISA, WB
• Application notes: ELISA:Sandwich:To detect Rat IL-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat IL-4 (XP-5190) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-4.Western Blot:To detect rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Il4e12, Il-4, Interleukin-4, B-cell IGG differenti
• Research Area: Immunology & Inflammation
• Note: For research use only
• NCBI: P20096

To detect Rat IL-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Rat IL-4 (orb1272452) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-4.

To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.