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Catalog Number | orb1272817 |
---|---|
Category | Antibodies |
Description | IL-33 Antibody [IL33305B] |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | IL33305B |
Tested applications | ELISA, FA, IHC, IP, WB |
Reactivity | Human |
Isotype | IgG2aκ |
Immunogen | Recombinant human IL-33. |
Antibody Type | Primary Antibody |
Concentration | 1 mg/ml |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. |
Alternative names | Interleukin-33; IL-1F11; NF-HEV Read more... |
Note | For research use only |
Application notes | ELISA: (direct or indirect: 1:2'000-1:5'000), Functional Application (Blocking), Immunohistochemistry (paraffin sections (1:50-1:200)), Immunoprecipitation Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of human tissue using anti-IL-33 (human), mAb (IL33305B) (Prod. No. orb1272817) at 1:100 dilution. A. Immunoperoxidase staining (cytoplasmic) of formalin-fixed, paraffin-embedded human heart (100x, brown colour). B. Isotype control with IgG2a (negative control)
Inhibition of binding of recombinant human IL-33 (hIL-33) to human ST2 (hST2) in vitro using anti-IL-33 (human), mAb (IL33305B) (Prod. No. orb1272817). An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml) ; 2) add 2 ug/ml of IL-33 with or without a hIL-33 mAb to thewells followed by washing; 3) add anti-FLAG HRP conjugated (1:2000) to an enzyme; 4) after adding the TMB solution, incubate at RT in the dark for 20 minutes. Immediately read the plate at 450 nm
Immunoprecipitation of recombinant hIL-33 proteins using anti-IL-33 (human), mAb (IL33305B) (orb1272817). Recombinant hIL-33 Proteins were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot/ECL reaction with anti-FLAG or anti-His.
Western blot analysis of human IL-33 using anti-IL-33 (human), mAb (IL33305B) (orb1272817) at 1:2000 dilution. 1. Recombinant human IL-33 (His tagged). 2. Recombinant human IL-33 (FLAG?-tagged). 3. Recombinant mouse IL-33 (FLAG?-tagged). 4. Other protein (His tagged, negative control).
ELISA, FA, IHC, IP, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |