IFNL1 Antibody

SKU: orb345149

Description

IL-29 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:10,000, WB: 1:1000
• Reactivity: Human
• Application Notes:
  This purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.9 kDa in size corresponding to the mature human IL-29 protein by western blotting in appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human IL-29 protein produced in E.coli.
• Target: IFNL1
• Purity: Anti-IL-29 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-29 protein. A BLAST analysis was used to suggest cross-reactivity with IL-29 from human sources based on 100% homology with the immunizing sequence. Based on high to 100% homology, there is a chance of cross-reactivity to IL-29 from a wide variety of animals. Cross-reactivity with IL-29 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store anti-IL-29 antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-IL-29 antibody, rabbit anti-Interferon lambda-1 antibody, rabbit anti-IFN-lambda-1 antibody, Interferon lambda-1, Cytokine Zcyto21, Interleukin-29, IL-29, IFNL1, IL29, ZCYTO21

Anti-human IL-29 antibody in western blot shows detection of recombinant human IL-29 raised in E.coli. Recombinant protein (0.1 µg, 19.9 kDa) was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Biorbyt's Anti-Human IL-29. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20000 in blocking buffer (p/n orb348637) for 30 min at RT.

Quick Database Links

Gene Symbol

IFNL1

UniProt

Q8IU54

RefSeq (Protein):NP_742152.1