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Catalog Number | orb345149 |
---|---|
Category | Antibodies |
Description | IL-29 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human IL-29 protein produced in E.coli. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:10,000, WB: 1:1000 |
Form/Appearance | Lyophilized |
Purity | Anti-IL-29 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-29 protein. A BLAST analysis was used to suggest cross-reactivity with IL-29 from human sources based on 100% homology with the immunizing sequence. Based on high to 100% homology, there is a chance of cross-reactivity to IL-29 from a wide variety of animals. Cross-reactivity with IL-29 from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q8IU54 |
NCBI | NP_742152.1 |
Storage | Store anti-IL-29 antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | None |
Alternative names | rabbit anti-IL-29 antibody, rabbit anti-Interferon Read more... |
Note | For research use only |
Application notes | This purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.9 kDa in size corresponding to the mature human IL-29 protein by western blotting in appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of recombinant protein (0.1 ug, 19.9 kDa) was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose using IL-29 antibody
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