IL21 Antibody

SKU: orb344651

Description

IL21 antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:6,000, WB: 1 µg/mL
• Reactivity: Bovine, Equine, Porcine
• Application Notes:
  IL-21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes, and in HL-60 and THP-1 cell lines. This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15.1 kDa in size corresponding to bovine IL-21 protein by western blotting in the appropriate cell lysate or extract.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to amino acid residues 24-152 of bovine IL-21 protein.
• Target: IL21
• Purity: This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine IL-21 protein. A BLAST analysis was used to suggest cross-reactivity with IL-21 from bovine sources based on 100% homology with the immunizing sequence. Based on 92% homology, there is a chance of cross-reactivity to porcine IL- 21, 91% to horse IL-21, 89% to human and dog, 88% to macaque, 84% to platypus and Syrian hamster, 76-79% to mouse, 75% to rat, and 48% to chicken. Cross-reactivity with IL-21 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-IL21 Antibody, Interleukin-21, IL-21, Interleukin21, Interleukin 21, IL 21

Quick Database Links

Gene Symbol

IL21

UniProt

Q76LU5

RefSeq:Q76LU5.2