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Catalog Number | orb1229838 |
---|---|
Category | Proteins |
Description | IL-1RA Recombinant Protein |
Tag | Tag Free |
Form/Appearance | Lyophilized |
Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
MW | 17.26 kD |
Target | IL1RN |
UniProt ID | P18510 |
Protein Sequence | Arg26-Glu177 |
Source | E. coli |
Biological Activity | ED50 is 0.5 ng/ml as determined by the dose-dependent inhibition of IL-1 stimulation of D10S cells.Specific Activity of 2.0 x 106 IU/mg. |
NCBI | P18510 |
Storage | Lyophilized protein should be stored at -20˚°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7˚°C for 2-7 days.Aliquots of reconstituted samples are stable at -20˚°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 50mM TrisHCl, 200mM NaCl, pH 7.5. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
Alternative names | Interleukin-1 Receptor Antagonist Protein, IL-1RN, Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for biological assays. For research use only. |
Expiration Date | 6 months from date of receipt. |
> 95 % by SDS-PAGE and HPLC analyses. | |
Approximately 16.8 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids. | |
Escherichia coli |
> 96% as determined by SDS-PAGE and HPLC. | |
17.1 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
17.3 kDa | |
E.Coli |
> 95% as determined by SDS-PAGE and HPLC. | |
17.4 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
17.26 kDa | |
E.coli |