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IL-17F Antibody Peroxidase Conjugated
Catalog Number: orb345229
| Catalog Number | orb345229 |
|---|---|
| Category | Antibodies |
| Description | IL-17F antibody (Peroxidase) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC, WB |
| Reactivity | Mouse |
| Isotype | IgG |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL17-F protein. |
| Antibody Type | Primary Antibody |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000 |
| Form/Appearance | Lyophilized |
| Purity | This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse IL17-F in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended. |
| Conjugation | HRP |
| UniProt ID | Q7TNI7 |
| NCBI | NP_665855.2 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Alternative names | rabbit anti-IL-17F peroxidase conjugated antibody, Read more... |
| Note | For research use only |
| Application notes | This purified antibody has been tested in western blotting and suitable in ELISA. By western blot a band approximately 17.9 kDa in size corresponding to mouse IL17-F protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. |
| Expiration Date | 12 months from date of receipt. |
Western Blot of Peroxidase Conjugated Rabbit anti-IL-17F antibody. Lane 1: Mouse IL-17F. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 16 kDa, 16 kDa for Mouse IL-17F. Other band(s): None.
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