IL-17D Antibody

• Catalog Number: orb1248053
• Category: Antibodies
• Description: IL-17D Antibody
• Target: IL-17D
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-EKDADSINSSIDK
• UniProt ID: Q8TAD2
• MW: Approx 28kDa
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 28kDa band observed in Human Adipose lysates (calculated MW of 21.9kDa according to NP_612141.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IL17D, interleukin 17D, IL-17D, IL-22, IL-27, IL27
• Note: For research use only
• NCBI: NP_612141.1

orb1248053 (0.3 ug/ml) staining of Human Adipose lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248053 (5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.