IL-10 rabbit pAb

• Catalog Number: orb766743
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IL10
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human IL10. AA range:71-120
• MW: 20
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-IL10 antibody, anti-Interleukin-10 antibody,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of HepG2, Mouse liver cells using IL-10 Polyclonal Antibody. Secondary antibody was diluted at 1:20000.

Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:100.

Immunohistochemical analysis of paraffin-embedded Human Cerebral cortex. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).