IKBKE Antibody

SKU: orb1239458

Description

IKBKE Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-IKK epsilon antibody (orb1239458) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IKK epsilon. The immunogen is located within the last 50 amino acids of IKK epsilon.
• Target: IKBKE
• Molecular Weight: Predicted: 80kD, Observed: 80 kD
• Purification: IKK epsilon Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures
• Form/Appearance: Liquid
• Buffer/Preservatives: IKK epsilon Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IKK epsilon Antibody: IKKE, IKKI, IKK-E, IKK-i, IKKE, KIAA0151, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, Inducible I kappa-B kinase, I-kappa-B kinase epsilon

Induction Validation in Mouse Cell Line. Loading: 15 µg of Raw264.7 cell lysates per lane. Antibodies: IKK epsilon orb1239458 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Cells were treated with LPS (0.3 µg/mL) for 3 hrs (lane 2) and 6 hrs (lane 3) or with control (lane 1).

Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: IKK epsilon orb1239458 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Tissue Lysates. Loading: 15 µg of lysates per lane. Antibodies: IKK epsilon orb1239458 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Jurkat Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: IKK epsilon orb1239458 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of IKK epsilon in Human Pancreas Tissue. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IKK epsilon antibody (orb1239458) at 10 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of IKK epsilon in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling IKK epsilon with orb1239458 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Quick Database Links

Gene Symbol

IKBKE

UniProt

Q14164

RefSeq:AF241789