IKK beta antibody

• Catalog Number: orb556271
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IKK beta
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human IKK beta. The exact sequence is proprietary.
• Concentration: 0.41 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 87
• Target: IKK beta
• UniProt ID: O14920
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.01% Thimerosal. 0.1M Tris-Glycine (pH7), 10% Glycerol
• Alternative names: inhibitor of nuclear factor kappa B kinase subunit
• Note: For research use only
• Application notes: Positive control: DDDDK-human IKBKB-transfected 293T , PC-12 , A431 , HeLa , HepG2
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of A431 cells using IKK beta antibody

Immunohistochemical staining of human colon carcinoma using IKK beta antibody

Western blot analysis of Various whole cell using IKK beta antibody

Western blot analysis of Whole cell extract using IKK beta antibody

Western blot analysis of HeLa(Lane 1), Hep G2(Lane 2), MOLT4(Lane 3) using HCE antibody