CHUK Antibody

SKU: orb1258311

Description

CHUK Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 650 to the C-terminus of human IKK alpha (NP_001269.3).
• Target: CHUK
• Molecular Weight: Observed: 94kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IKK alpha Antibody: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase alpha

Western blot analysis of extracts of various cell lines, using IKK alpha antibody (orb1258311) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat brain using IKK alpha antibody (orb1258311) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using IKK alpha antibody (orb1258311) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using IKK alpha antibody (orb1258311). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of 293T cells using 3 ug IKK alpha antibody (orb1258311). Western blot was performed from the immunoprecipitate using IKK alpha antibody (orb1258311) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

CHUK

UniProt

O15111