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Catalog Number | orb526702 |
---|---|
Category | Antibodies |
Description | IKB alpha Mouse Monoclonal Antibody |
Target | NFKBIA |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | IgG1 |
Conjugation | Unconjugated |
Reactivity | Human, Rat |
Predicted Reactivity | Mouse |
Form/Appearance | Liquid |
Concentration | 1mg/ml |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Immunogen | Recombinant human IKB alpha Protein (1-77/314aa) |
UniProt ID | P25963 |
MW | 36 kDa |
Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ICC/IF=1:50-1:200 |
Antibody Type | Primary Antibody |
Clone Number | 2A5 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | NFKBIA; Inhibitor of KB alpha; I kappa B alpha; I( Read more... |
Note | For research use only |
Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IKB alpha) Monoclonal Antibody, Unconjugated (orb526702) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-IKB alpha (orb526702) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 36 kD.
Sample: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-IKB alpha (orb526702) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 36 kD.
Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Lane 3: Siha (Human) Cell Lysate at 30 ug, Primary: Anti-IKB alpha (orb526702) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 39 kD, Observed band size: 39 kD.
ICC, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |