IKB alpha Antibody, KO Validated

• Catalog Number: orb1274539
• Category: Antibodies
• Description: IKB alpha Antibody, KO Validated
• Target: NFKBIA
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human IKB alpha (NP_065390.1).
• UniProt ID: P25963
• MW: Observed: 36kDa
• Tested applications: IP, WB
• Application notes: WB: 1:500 - 1:2000IP: 1:20 - 1:50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NFKBIA, IKBA, MAD-3, NFKBI
• Note: For research use only

Western blot analysis of extracts of various cell lines, using IKB alpha antibody (orb1274539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts from normal (control) and IKB alpha knockout (KO) 293T cells, using IKB alpha antibody (orb1274539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunoprecipitation analysis of 150 ug extracts of A549 cells using 3 ug IKB alpha antibody (orb1274539). Western blot was performed from the immunoprecipitate using IKB alpha antibody (orb1274539) at a dilution of 1:500.