IgM Heavy Chain Antibody

• Catalog Number: orb248394
• Category: Antibodies
• Description: This antibody recognizes a protein of 75kDa, identified as the mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Recombinant heavy chain of human IgM was used as the immunogen for this antibody.
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1ug/ml, Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: IM373
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the IgM heavy chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This IgM heavy chain antibody is available for research use only.
• Note: For research use only
• Entrez: 3507

IHC testing of human tonsil stained with IgM heavy chain antibody.

IHC testing of human tonsil stained with IgM heavy chain antibody.

Western blot testing of Raji cell lysate with IgM heavy chain antibody. Expected molecular weight: 70-75 kDa.

SDS-PAGE Analysis of Purified, BSA-Free IgM Heavy Chain Antibody (clone IM373). Confirmation of Integrity and Purity of the Antibody.