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IgM Heavy Chain Antibody

Catalog Number: orb248394

DispatchUsually dispatched within 5-10 working days
$ 764.00
Catalog Numberorb248394
CategoryAntibodies
DescriptionThis antibody recognizes a protein of 75kDa, identified as the mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Species/HostMouse
ClonalityMonoclonal
Clone NumberIM373
Tested applicationsFACS, IF, IHC-P, WB
ReactivityHuman
IsotypeMouse IgG2b, kappa
ImmunogenRecombinant heavy chain of human IgM was used as the immunogen for this antibody.
Dilution rangeFlow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1ug/ml, Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis IgM heavy chain antibody is available for research use only.
Entrez3507
StorageStore the IgM heavy chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
IgM Heavy Chain Antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human tonsil tissue using IgM Heavy Chain antibody

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