IGHA1 Antibody (C-term)

SKU: orb1929242

Description

IGHA1 Antibody (C-term)

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution range: WB - 1:2000, IHC-P - 1:100-500, FC - 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 37655 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Western blot analysis of IGHA1 antibody (C-term) in CEM cell line lysates (35 ug/lane). IGHA1 (arrow) was detected using the purified Pab.

Flow cytometric analysis of CEM cells using IGHA1 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-IgA Antibody (C-term) at 1:1000 dilution + A2058 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 38 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human Lymph reacted with IGHA1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-IGHA1 Antibody (C-term) at 1:2000 dilution. Lane 1: THP-1 whole cell lysate. Lane 2: Human plasma lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 55 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P01876