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Catalog Number | orb385736 |
---|---|
Category | Antibodies |
Description | Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | IG217 |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | Purified human Ig Gamma Chain was used as the immunogen for this IgG antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1) (2) |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This IgG antibody is available for research use only. |
UniProt ID | P01860, P01857, P01859, P01861 |
Storage | Store the IgG antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The optimal dilution of the IgG antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Human tonsil using IgG antibody
ELISA, WB | |
Rabbit | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC, WB | |
Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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