IGHG1, IGHG2, IGHG3, IGHG4 Antibody

SKU: orb1252380

Description

IGHG1, IGHG2, IGHG3, IGHG4 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (Formalin/paraffin) 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The optimal dilution of the IgG antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: IG217
• Immunogen: Purified human Ig Gamma Chain was used as the immunogen for this IgG antibody.
• Target: IGHG1, IGHG2, IGHG3, IGHG4
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Formalin-fixed, paraffin-embedded human tonsil stained with IgG antibody (IG217).

SDS-PAGE Analysis of Purified, BSA-Free IgG Antibody (clone IG217). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

IGHG1, IGHG2, IGHG3, IGHG4

UniProt

P01860, P01859, P01861, P01857