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Catalog Number | orb1260913 |
---|---|
Category | Antibodies |
Description | IGFBP5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 68-167 of human IGFBP5 (NP_000590.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 30kDa |
Target | IGFBP5 |
UniProt ID | P24593 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | IBP5, IBP5, Insulin-like growth factor-binding pro Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using IGFBP5 antibody (orb1260913) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse brain using IGFBP5 antibody (orb1260913) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using IGFBP5 antibody (orb1260913) at dilution of 1:100 (40x lens).
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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