You have no items in your shopping cart.
Cart summary
Item 1 of 3
Item 1 of 3
IGFBP4 Antibody (N-term)
Catalog Number: orb1929696
| Catalog Number | orb1929696 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This IGFBP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-92 amino acids from the N-terminal region of human IGFBP4. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Porcine |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | P22692 |
| MW | 27934 Da |
| Tested applications | FC, WB |
| Dilution range | WB: 1:2000, WB: 1:2000, FC: 1:25 |
| Antibody Type | Primary Antibody |
| Clone Number | RB20479 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Insulin-like growth factor-binding protein 4, IBP- Read more... |
| Note | For research use only |
| NCBI | NP_001543.2 |
Anti-IGFBP4 Antibody (N-term) at 1:2000 dilution + Rat ovary lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-IGFBP4 Antibody (N-term) at 1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Mouse ovary lysate. Lane 3: Rat ovary lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing A549 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
