IGFBP1 Antibody

SKU: orb1248490

Description

IGFBP1 Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 30kDa band observed in Human Placenta lysates (calculated MW of 27.9kDa according to NP_000587.1). Recommended concentration: 1-3µg/mL. An additional band of 55kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-STYDGSKALHVTNIK
• Target: IGFBP1
• Molecular Weight: Approx 30kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IGFBP1, insulin-like growth factor binding protein 1, AFBP, IBP1, IGF-BP25, PP12, hIGFBP-1, IBP-1, IGF-binding protein 1, IGFBP-1, OTTHUMP00000159561, OTTHUMP00000207990, OTTHUMP00000214348, alpha-pregnancy-associated endometrial globulin, amniotic fluid binding protein, binding protein-25, binding protein-26, binding protein-28, growth hormone independent-binding protein, insulin-like growth factor-binding protein 1, placental protein 12

orb1248490 (2 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248490 (10 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

IGFBP1

UniProt

P08833

RefSeq (Protein):NP_000587.1